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Paper title Fluorescence Angiography for Evaluation of Aneurysm Perfusion and Parent Artery Patency in a Rabbit Sidewall Aneurysm Model: Technical Note
Paper code P38
Authors
  1. Fabio von Faber-Castell UniversitätsSpital & Universität Zürich Speaker
  2. Basil E. Grüter Kantonsspital Aarau Speaker
  3. Sivani Sivanrupan Cerebrovascular Research Group, University of Bern
  4. Javier Fandino Kantonsspital Aarau
  5. Serge Marbacher Kantonsspital Aarau
Form of presentation Poster
Topics
  • Joint SSNR | SSNS
Abstract text Background
Brain aneurysm treatment focuses mainly on complete occlusion without any residual neck. Parent arterial flow should be patent. In neurosurgery various techniques are used to control the blood flow. Techniques are Doppler ultrasonography, electrophysiological monitoring and common cerebral angiography. Furthermore, other studies also mention fluorescein sodium and indocyanine green (ICG) as visualization methods. Only a few publications on fluorescein videoangiography (FVA) are available. FVA was first introduced in neurosurgery in 1967 and was used to check the epicerebral blood circulation in therapeutic craniotomy.
The authors want to verify the real-time blood perfusion and occlusion of the aneurysm after inducing aneurysms in rabbits with FVA as an easy to use, reliable and inexpensive Method.

Methods
Twenty sidewall aneurysms were created in ten rabbits by suturing an arterial vessel pouch on the carotid artery of a donor rabbit. One month after induction surgery the aneurysms were controlled for patency. The artery was dissected and Fluoresceine Faure (10%, 1ml) was injected intravenously via catherization of the ear vein. (The perfusion of the dye was recorded with a video camera.) Aneurysms were harvested and the patency was controlled macroscopically followed by histologically examination.

Results
All but one occlusions and patency diagnosed via fluorescein angiography could be confirmed macroscopically. Fluorescein video angiography was feasible in 16/20 aneurysms and showed n=12 cases with patent parent artery and n=4 without flow at follow-up. The perfusion of the dye was recorded. No complications occurred. A catheter allows injection of more than one substance at the same time. Venous injections have less complications than arterial and the risk of contamination of the surgical field is lower in peripheral injections.

Conclusion
Fluorescein is cheap and easily applicable and no special equipment is needed. This is a safe and very effective method for evaluating in-vivo perfusion status in an experimental setting with rabbits. Fluorescence videoangiography with fluorescein as contrast agent seems to be effective to control patency of aneurysms and underlying vessel or in bypass surgery. It gives a real-time picture of the patency and can be done without expensive equipment during surgery.